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293t platinum-e retroviral packaging cell line (plat-e  (Cell Biolabs Inc)

 
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    Structured Review

    Cell Biolabs Inc 293t platinum-e retroviral packaging cell line (plat-e
    Transfection reagents Lipofectamine 2000, Lipofectamine LTX, TransIT-293, TransIT-2020, TransIT-LT1, PolyJet, or LipoJet, were used for pMX-GFP <t>retroviral</t> vector transfection. A. GFP expression <t>in</t> <t>Plat-E</t> cells 2 days after transfection by each transfection reagent, and following infection to primary myoblasts with indicated viral supernatants for 2 days. These phase contrast images show primary myoblasts after infection. C. The number of GFP positive primary myoblasts out of total cells. Data are presented as the mean ± SEM. (n=3)
    293t Platinum E Retroviral Packaging Cell Line (Plat E, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/293t platinum-e retroviral packaging cell line (plat-e/product/Cell Biolabs Inc
    Average 90 stars, based on 1 article reviews
    293t platinum-e retroviral packaging cell line (plat-e - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Spin infection for efficient gene delivery in muscle stem cells for intramuscular cell transplantation"

    Article Title: Spin infection for efficient gene delivery in muscle stem cells for intramuscular cell transplantation

    Journal: BioTechniques

    doi: 10.2144/000114576

    Transfection reagents Lipofectamine 2000, Lipofectamine LTX, TransIT-293, TransIT-2020, TransIT-LT1, PolyJet, or LipoJet, were used for pMX-GFP retroviral vector transfection. A. GFP expression in Plat-E cells 2 days after transfection by each transfection reagent, and following infection to primary myoblasts with indicated viral supernatants for 2 days. These phase contrast images show primary myoblasts after infection. C. The number of GFP positive primary myoblasts out of total cells. Data are presented as the mean ± SEM. (n=3)
    Figure Legend Snippet: Transfection reagents Lipofectamine 2000, Lipofectamine LTX, TransIT-293, TransIT-2020, TransIT-LT1, PolyJet, or LipoJet, were used for pMX-GFP retroviral vector transfection. A. GFP expression in Plat-E cells 2 days after transfection by each transfection reagent, and following infection to primary myoblasts with indicated viral supernatants for 2 days. These phase contrast images show primary myoblasts after infection. C. The number of GFP positive primary myoblasts out of total cells. Data are presented as the mean ± SEM. (n=3)

    Techniques Used: Transfection, Plasmid Preparation, Expressing, Infection



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    Cell Biolabs Inc 293t platinum-e retroviral packaging cell line (plat-e
    Transfection reagents Lipofectamine 2000, Lipofectamine LTX, TransIT-293, TransIT-2020, TransIT-LT1, PolyJet, or LipoJet, were used for pMX-GFP <t>retroviral</t> vector transfection. A. GFP expression <t>in</t> <t>Plat-E</t> cells 2 days after transfection by each transfection reagent, and following infection to primary myoblasts with indicated viral supernatants for 2 days. These phase contrast images show primary myoblasts after infection. C. The number of GFP positive primary myoblasts out of total cells. Data are presented as the mean ± SEM. (n=3)
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    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Transfection reagents Lipofectamine 2000, Lipofectamine LTX, TransIT-293, TransIT-2020, TransIT-LT1, PolyJet, or LipoJet, were used for pMX-GFP retroviral vector transfection. A. GFP expression in Plat-E cells 2 days after transfection by each transfection reagent, and following infection to primary myoblasts with indicated viral supernatants for 2 days. These phase contrast images show primary myoblasts after infection. C. The number of GFP positive primary myoblasts out of total cells. Data are presented as the mean ± SEM. (n=3)

    Journal: BioTechniques

    Article Title: Spin infection for efficient gene delivery in muscle stem cells for intramuscular cell transplantation

    doi: 10.2144/000114576

    Figure Lengend Snippet: Transfection reagents Lipofectamine 2000, Lipofectamine LTX, TransIT-293, TransIT-2020, TransIT-LT1, PolyJet, or LipoJet, were used for pMX-GFP retroviral vector transfection. A. GFP expression in Plat-E cells 2 days after transfection by each transfection reagent, and following infection to primary myoblasts with indicated viral supernatants for 2 days. These phase contrast images show primary myoblasts after infection. C. The number of GFP positive primary myoblasts out of total cells. Data are presented as the mean ± SEM. (n=3)

    Article Snippet: Retroviral supernatants were produced by transfection of pMX-GFP (Cell Biolabs, San Diego, CA, USA) or a pMX-mCherry retroviral vector into a 293T Platinum-E Retroviral Packaging Cell Line (Plat-E) (Cell Biolabs).

    Techniques: Transfection, Plasmid Preparation, Expressing, Infection